Enumeration of CD34+ haemopoietic stem cells: comparative study of the performance of the SYSMEX XN-1000 hematology analyzer in a dual-platform approach versus a single-platform approach

Data regarding the quantification of CD34+ hematopoietic stem cell HSC in dual-platform (DP) approach using a hematological analyzer featuring dedicated nRBC channel are limited if not inexistent. The objective this study is to compare performance DP on new generation channel, Sysmex XN-1000, with single-plate (SP) form Beckman Coulter’s Navios flow cytometer. Using International Society for Hematotherapy and Transplantation protocol, total 86 samples were analyzed multiple myeloma patients planned autologous transplantation. Stem-Kit™ reagents used perform both approaches SP. count cells SP deduced directly from software that manage cytometry was compared obtained by DP. made combining analysis XN-1000 data. agreement results between these two Bland-Altman diagram gave bias one cell/μl. As equation right Passing-Bablok, it Y = 0.166667 + 0.969697 X (DP). slight differences counting did achieve statistical significance. Both effective yield similar results. seems be simple affordable well suited needs laboratories where type available.